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Sftpc creert2

Web7 Oct 2024 · To assess the relative contributions of AT2 and AT1 cells to tissue regeneration after hyperoxic injury, we lineage traced AT2 and AT1 cells using the SftpcCreERT2;R26REYFP and HopxCreERT2;R26REYFP alleles, respectively. Web5 May 2024 · To explore this possibility we lineage-labeled AT2 cells and BASCs using an Sftpc CreERT2;mTmG mouse line. We found that labeling the BASCs required high doses …

Age-dependent alveolar epithelial plasticity orchestrates lung ...

Web7 Oct 2024 · To assess the relative contributions of AT2 and AT1 cells to tissue regeneration after hyperoxic injury, we lineage traced AT2 and AT1 cells using the … WebSftpcCreERT2/+;tdTomatoflox/flox mice were used for the labelling of AT2 cells and labelled subpopulations were analysed by flow cytometry, quantitative PCR, assay for transposase-accessible chromatin using sequencing (ATAC-seq), gene arrays, pneumonectomy and culture of precision-cut lung slices. cute sayings to say to your crush https://aeholycross.net

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Web1 Feb 2024 · SFTPC-CreERT2;R26ReYFP at 7 days of culture. Membrane in magenta, SFTPC in green and nuclei in grey. Why I like this preprint. 3D cultures of AT2 cells are currently being used more and more for disease modeling of lung diseases involving the alveoli. From lung adenocarcinoma to COVID, there are multiple deadly diseases that use AT2 ... WebUsing the Sftpc CreERT2; tdTomato lineage-traced mice, we recently reported a novel AT2-IAAP subpopulation characterized by low Sftpc expression but that is enriched for PD-L1 expression . IAAP cells, which represent quiescent and immature epithelial progenitors, undergo activation and expansion following PNX injury . Web008179 B6.129S4- Kras tm4Tyj /J This Kras LSL-G12D strain carries a Lox-Stop-Lox (LSL) sequence followed by the K-ras G12D point mutation allele commonly associated with … cute sayings to put on dog shirts

Early lineage specification defines alveolar epithelial ontogeny in …

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Sftpc creert2

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WebSftpc CreERT2/+;tdTomato flox/flox mice were used for the labelling of AT2 cells and labelled subpopulations were analysed by flow cytometry, quantitative PCR, assay for … Web19 Oct 2024 · The Sftpc-CreERT2 mouse line has been used previously to track AT2 cell fate (65, 66). Gramd2-CreERT2 mice were generated by homologous recombination insertion …

Sftpc creert2

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WebUsing Sftpc CreERT2/+ : tdTomato flox/flox mice, we previously identified a lung populat... View Identification of a novel subset of alveolar type 2 cells enriched in PD-L1 and expanded following ... WebMouse Lines Sftpc Creert2, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

Web9 Mar 2024 · To specifically confirm which Fgfrs are expressed in mouse AEC2s, we combined the SPC + AEC2-specific Cre recombinase (Sftpc CreERT2) with the ROSA mTmG (mT/mG) Cre reporter (Sftpc CreERT2;ROSA mT/mG control mice). At 6 weeks of age, these mice were administered intraperitoneal tamoxifen, and 2 weeks after tamoxifen … http://www.informatics.jax.org/allele/MGI:5465199

Web6 Feb 2024 · However, tamoxifen-treated Sftpc CreERT2; hACE2 fl/y males infected with SARS-CoV-2 virus exhibited levels of viral nucleocapsid protein and viral RNA in the lung … Web15 Jun 2024 · The total AT2 population (Sftpc Pos Tom Pos cells) was isolated from lungs of 6-8 weeks old Sftpc CreERT2/+; tdTomato flox/flox mice. For the single-cell …

Web( C ) Adult Sftpc CreERT2 or Sftpc CreERT2 ; Rosa26 miR-302 mice received two doses of tamoxifen. AT2 cells were purified after 7 days from last tamoxifen treatment, and expression of Micu1 was examined by qPCR. Gene expression was normalized to Rps13 , a mitochondrial gene. ( D ) Transmission electron microscopy of lungs 3 weeks after ...

Web28 Aug 2024 · Upon uptake, sLP-mCherry fluorescence has an intracellular half-life of 43h ( Extended Data Figure 1f) and is localized in CD63 + multi-lamellar bodies (lysosomal-like structures), where, due to its high photostability 5, it retains high fluorescent intensity ( Extended Data Figure 1g, h ). cheap boots for women irelandWeb1 Feb 2024 · To investigate the function of Wnt signaling, we deleted β-catenin, a transducer of canonical Wnt pathway activity, in mature AT2 cells by using Lyz2-Cre or SftpC-CreERT2 while simultaneously marking recombined cells by using Rosa26mTmG. We reasoned that only cells with active Wnt signaling (Axin2 + AT2 cells) would be affected. cheap boots for women clearanceWebTmx-inducible AT2 cell-specific Grp78 KO mice, referred to as Grp78SCE (genotype Sftpc+/creERT2;Grp78f/f; Cre is heterozygous) or Grp78hSCE ( genotype SftpccreERT2/creERT2;Grp78f/f; Cre is homozygous), respectively, and corresponding reporter mice, Grp78SCE; ROSAtm/tm and Grp78hSCE; ROSAtm/tm ( 25 ), were generated … cute saying sweatshirtsWebUsing cell state trajectory modeling, guided by RNA velocity of single cells from 18 timepoints and lineage tracing Sox2-CreERT2 and Sftpc-CreERT2 mice, we show that … cute sayings to write in baby booksWeb丁香通为您提供Arl8aFlox商品详情介绍：价格：询价，货号：NM-CKO-2110046，品牌：南模生物，产地：中国上海，详见丁香通Arl8aFlox商品详情页； cheap boots for boysWebAlveolar type II (AT2) cell dysfunction contributes to a number of significant human pathologies including respiratory distress syndrome, lung adenocarcinoma, and … cheap boots for women size 10Web21 Sep 2024 · Surprisingly, when Tmx was injected after viral infection, we observed 7.9% ± 1.4% Trp63 CreERT2 lineage-labeled cells expressed the AT2 cell marker SftpC but not p63 or Krt5 in the areas that surrounded the injured foci, and the number was increased to 19.4%±4.2% in Trp63 CreERT2;Trpm5-/-;R26 Ai14 mice (Figure 5A and Figure 5—figure ... cheap boots for womens for under 20 dollars