Hieffclone
Web翌圣生物以工具酶为核心,研发生产销售pcr,qpcr,高保真酶,热启动酶,一步法快速克隆试剂盒,一步法快速支原体检测试剂盒等分子、蛋白、细胞、免疫等3000多种试剂及试剂盒,现有科研客户数量多达1.2万家。 WebCN114134170A CN202411457529.2A CN202411457529A CN114134170A CN 114134170 A CN114134170 A CN 114134170A CN 202411457529 A CN202411457529 A CN 202411457529A CN 114134170 A CN114134170 A CN 114134170A Authority CN China Prior art keywords mcs product pgl primer nos Prior art date 2024-12-02 Legal status …
Hieffclone
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Web16 de dez. de 2024 · The linear DNA was cyclized into ΔKlcA HS pHS10842 using the HieffClone One Step Cloning kit (Shanghai Yeasen Biotech Co., Ltd., Shanghai, China) … WebHieffClone®ZeroTOPO-TASimpleCloningKit不含多克隆酶切位点(MCS),在引入后续酶切位点进行酶切操作时,不会受到 MCS位点的影响,从而增加酶切效率以及克隆成功率。 …
Web6 de mar. de 2024 · The cloning backbone PL451 and the three segments of CVA10 carrying overlapping end (15–20 bp) were amplified and seamlessly linked together in the transformed E. coli by recombination according to HieffClone TM MultiOneStep Cloning kit (YEASEN, Shanghai, China).
Web15 de dez. de 2024 · Avian leukosis virus subgroup J disease (ALV-J) is a contagious and immunosuppressive avian disease caused by ALV-J virus. Although miRNA participate in various biological processes of tumors, little is known about the potential role of miRNA in ALV-J. Our previous miRNA and RNA sequencing data showed that the expression of … Web29 de jun. de 2024 · The DNA fragment of TurboID was cloned into this digested vector by a HieffClone Plus multi One-Step Cloning Kit (10912ES10, Yesen, China). The TurboID …
WebHieff Clone ® Plus One Step Cloning Kit 是一款简便、快速、高效的 DNA定向克隆产品,该试剂盒可以将PCR产物定向克隆至任何载体的任何位点, 可高效克隆 50 bp-10 kb片段。. 将载体线性化,并在插入片段正、反 …
Web1 de dez. de 2024 · The enzymes using in molecular cloning experiment were Sap Ⅰ (NEB, USA), T4 DNA Ligase (Takara, Japan) and 2 × Hieffclone Enzyme Mix (YEASEN, … greensboro mitsubishiWeb丁香通为您提供HieffClone™O商品详情介绍:价格:¥1 - 1680,货号:10905ES25,品牌:进口/国产,产地:德国/中国,详见丁香通HieffClone™O商品详情页; fmb114867Web产品描述. 本试剂盒是利用拓扑异构酶(Topoisomerase)高效快速连接DNA片段的原理进一步研发而成,与传统的T4连接酶相比,具有以下优势:1)快速,仅需1-5分钟即可完成 … fmb 120Web公司简介. 上海翊圣生物科技有限公司成立于2009年,总部设在上海,并且分别在北京、杭州、南京、武汉、广州(深圳)设有办事处。. 公司专注于酶制试剂和抗原抗体的开 … greensboro mobile veterinary housecallsWeb10 de abr. de 2024 · Hieff CloneTM Plus One Step Cloning Kit是一款简单、快速、高效的DNA定向克隆产品,该试剂盒可以将PCR产物定向克隆至任意载体的任意位点,可高效 … greensboro mlk day of serviceWeb12 de nov. de 2024 · 一种pbiflu荧光标签表达载体的制备方法及其应用 技术领域 1.本发明属于分子生物学技术领域,具体涉及一种pbiflu荧光标签表达载体的制备方法及其应用。 背景技术: 2.黄色荧光蛋白(yellow fluorescent protein,yfp)是将绿色荧光蛋白(gfp)第203为苏氨酸突变体为色氨酸,可以稳定生色团在激发状态的偶极矩 ... greensboro mls soccer teamWeb20 de jan. de 2024 · All plasmids were constructed using HieffClone One Step Cloning Kit (YEASEN, 10911). Point mutations of c-Myc (L297A, A321del, L333A, R346A, S347A, … fmb11an