WebJan 1, 2011 · FITC-dextran flux of HUVEC monolayers treated with VEGF. HUVEC were plated at confluent density for 3 days on Transwell filters. 1 mg/ml FITC-dextran (a) MW 10,000 or (b) MW 75,000 was placed in the upper well and cells were treated with 10 ng/ml VEGF for 15, 30, 60, and 120 min.A sample of medium from the lower chamber was … WebFITC-DEAE-Dextran 70 is a cationic fluorescent dextran that may be used to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-DEAE-Dextran 70 may be use to compare its selectivity versus neutral FITC-Dextran 70 or anionic FITC-CM-Dextran 70.
Intestinal permeability, digestive stability and oral …
WebJun 1, 2024 · Utilization of the FITC-d procedure as a marker for intestinal permeability is a useful tool that permits detection of leaky gut without requiring euthanasia. In contrast, measurement of bacterial translocation from the gut to peripheral organs necessitates euthanization and organ collection for further processing and culture ( Tellez et al ... WebJul 19, 2024 · Cellular permeability was investigated using a fluorescein isothiocyanate (FITC)-dextran permeability assay. FTY720, an analogue of sphingosine-1-phosphate (S1P), was tested for its potential ... great shepherd ministry
Establishment of a method for ... - Pediatric Research
WebAug 30, 2024 · In contrast, permeability of macromolecules measured at different time points as 4 kDA fluorescein isothiocyanate (FITC)-dextran flux across monolayers steadily decreased during this time. Detailed analysis suggested that this observation could be explained by alterations of junctional length along the cell borders within monolayers … WebFeb 20, 2008 · Vascular permeability was measured as the flux of FITC-labeled albumin from the circulation to the interstitial space as we and others have described , , using computer assisted imaging for quantification. There was a significant increase in permeability over time in response to MIP-2 superfusion in wild-type mice (Figure 1C). It … Web1 day ago · For assessment of the cell monolayer permeability, 1 mg/ml FITC-dextran (20 kDa) was introduced to the tight cell monolayers (TEER, approximately 3000 Ωcm 2) in the trans-wells for 30 min at room ... floral print shirred knotted top