WebAug 14, 2024 · # Run DIAMOND with blastx # Output format 6 produces a standard BLAST tab-delimited file $ {programs_array [diamond]} blastx \ --db $ {dmnd} \ --query "$ {transcriptomes_array [$fasta]}" \ --out "$ {transcriptome_name}" .blastx.outfmt6 \ --outfmt 6 \ --evalue 1e-4 \ --max-target-seqs 1 \ --block-size 15.0 \ --index-chunks 4 done
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WebSep 3, 2024 · hi, when you run TransDecoder.LongOrfs on the Trinity.fasta file, it should have created a directory: Trinity.fasta.transdecoder_dir Once you locate that directory, be sure to run the TransDecoder.Predict step in the working directory that contains that Trinity.fasta.transdcoder_dir/ within it. WebMar 25, 2024 · After the BLASTn, I followed that up by making a Krona plot using the taxonomic info pulled via BLASTn. This was run locally on my computer (swoose). Krona plot script: krona_tax_plots_blast.sh. #!/bin/env bash # Bash script for creating Krona plot of metagenomics taxonomies from BLAST outputs. # BLAST output format is expected to … iphone 7 plus cases leather
Table C1: [Options common to all BLAST+...]. - BLAST® Command …
WebMay 8, 2024 · # Run DIAMOND with blastx # Output format 6 produces a standard BLAST tab-delimited file $ {diamond} blastx \ --db $ {dmnd} \ --query "$ {fasta}" \ --out "$ … Web1. qseqid query or source (gene) sequence id. 2. sseqid subject or target (reference genome) sequence id. 3. pident percentage of identical positions. 4. length alignment … WebJan 23, 2024 · As part of annotating the transcriptome assembly from the MEGAN6 C.bairdi taxonomic-specific reads, I need to run DIAMOND BLASTx to use with Trinotate.. Ran DIAMOND BLASTx against the UniProt/SwissProt database (downloaded today) on Mox. SBATCH script (GitHub): 20240123_cbai_diamond_blastx_megan.sh iphone 7 plus is it waterproof