WebTo stain neutral lipids, cells were incubated with BODIPY (1:1,000; Thermo Fisher Scientific) for 1 h at 37 °C. Actin was stained with phalloidin (1:50; Invitrogen) for 30 min at RT and nuclei with DAPI (1:5,000; Thermo Fisher Scientific) for 15 min at RT. Samples were washed with PBS between steps. WebMar 23, 2024 · 2.11 BODIPY fluorescence staining For mice liver staining, liver tissue sections were frozen for 30 min and immersed in PBS for 10 min, and then soaked in BODIPY 493/503 (GLPBIO, GC42959, United States of America) for 30 min, protecting from light during staining.
Cholesteryl Bodipy Fl C12 Thermo Fisher Bioz
WebThe orange-red fluorescent fatty acid, BODIPY™ 558/568 C 12 can be used as a synthetic precursor to a wide variety of fluorescent phospholipids. Unlike fluorophores like pyrene, DPH and NBD, BODIPY™ dyes are relatively environment insensitive and is fluorescent in both aqueous and lipid environments. WebCholesterol is an abundant and important lipid component of cellular membranes. Analysis of cholesterol transport and diffusion in living cells is hampered by the technical … solina belgique
National Center for Biotechnology Information
WebThermo Fisher bodipy fl c 12 cholesterol ester. Bodipy Fl C 12 Cholesterol Ester, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based … WebNov 28, 2016 · For BODIPY staining, after 24 h of seeding, the BODIPY reagent was added and incubated in the incubator for 30 min. After incubation, cells were washed with 1X PBS and observed under the confocal microscope (497 nm excitation and 503 nm emission). Image-Pro and GraphPad prism software were used to quantify the images and analyze … WebUpon injection into the yolk sac, BODIPY-cholesterol did not disturb zebrafish development and was targeted to sterol-enriched brain regions in live fish. We conclude that this new probe closely mimics the membrane partitioning and trafficking of cholesterol and, because of its excellent fluorescent properties, enables the direct monitoring of ... solin adhésif